Biosynthesis Nanoparticles And Molecular Study To Detect Bacterial Isolated From Patients With Diabetic Foot Ulcers In Babylon Province

Objective:- Diabetic foot ulcers, the aim of study for determine some types microorganisms from diabetic ulcers thatcauses inflammation and long-term complications activity ZnO NPs biosynthesis by Aspergillus niger against isolatedbacteria, collected infected patients at general Al -Hashimiya hospital in Babylon Governorate.Methods:-collected 100 swabs, cultured it, bacteria was diagnostic morphological microscopic, standard biochemical tests andVitec,the isolates identified molecular diagnosis extraction DNA,using specific universal primer 16SrRNA (1239bp) andpolymerase chain reaction (PCR), biosynthesis,characterization zinc Oxide Nanoparticles to inhibited bacterial growth.Results:-The results were (26 aerobic positive) present such as Gram positive Staph aureus , regards aerobicGram negative bacilli, Klepsiella pneumoniae, Proteus mirabilis, Escherichia coli, The oxide nanoparticles created byAspergillus spherical shape with size 13.5nm that used antibacterial.Discussion:-Through determination MIC concentrations (31.25, 62.5μg /ml, 125μg/mL, 250μg/ml, 500μg /ml),(31.25, /ml) not prevent growth inhibition while 125μg/mL MIC, two best are 250μg/ml,500μg /ml both give zones.conclusion:- predominantly shown be a stabilizercapable producing nanoparticles, due is very suitable candidate carry out antimicrobial operation, result toits small size.